Western Blot; Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate; All lanes: INPP5D antibody at 1:1500; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 134, 110 kDa; Observed band size: 145 kDa
IHC image of RAC07732 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating SHIP in Raji whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07732 in Raji whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07732(2ug)+ Raji whole cell lysate(500ug); Lane 3: Raji whole cell lysate (10ug)