Western Blot; Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue; All lanes: ILK antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 52, 45, 37 kDa; Observed band size: 51 kDa
Immunofluorescence staining of MCF7 Cells with RAC07439 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ILK in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07439 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07439(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)