Western Blot, Positive WB detected in: Hela whole cell lysate (30ug), HepG2 whole cell lysate (30ug), U251 whole cell lysate (30ug), K562 whole cell lysate (30ug), colo205 whole cell lysate (30ug), A431 whole cell lysate (30ug), Jurkat whole cell lysate (30ug), SH-SY5Y whole cell lysate (30ug), NIH/3T3 whole cell lysate (30ug), All lanes: IMP 3 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 64 kDa, Observed band size: 69 kDa, Exposure time:20s
IHC image of RAC09129 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.