Western Blot; Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate; All lanes: Id1 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 17, 16 kDa; Observed band size: 26 kDa;
Immunofluorescence staining of Hela Cells with RAC07052 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).