ICAM1 Recombinant Monoclonal Antibody, Cat#RAC08080
Western Blot. Positive WB detected in:K562 whole cell lysate (20µg), A375 whole cell lysate (20µg). All lanes: ICAM1 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:57.83 kDa. Observed band size:58 kDa. Exposure time:120s
IHC image diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was human IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ICAM1 Recombinant Monoclonal Antibody, Cat#RAC08080 Western Blot. Positive WB detected in:K562 whole cell lysate (20µg), A375 whole cell lysate (20µg). All lanes: ICAM1 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:57.83 kDa. Observed band size:58 kDa. Exposure time:120s IHC image diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was human IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Ultra Sensitive IHC

ICAM1 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08080-100ul
Size: 100ul
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Western Blot. Positive WB detected in:K562 whole cell lysate (20µg), A375 whole cell lysate (20µg). All lanes: ICAM1 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:57.83 kDa. Observed band size:58 kDa. Exposure time:120s
IHC image diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing HepG2 cells stained at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was human IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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The peptide product is of exceptional quality and is highly recommended for its efficacy.

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I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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