HSPA8 Recombinant Monoclonal Antibody, Cat#RAC08144
Western Blot, Positive WB detected in: A549 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), U-251MG whole cell lysate (20ug), MCF-7 whole cell lysate (20ug), K562 whole cell lysate (20ug), HepG2 whole cell lysate (20ug), HeLa whole cell lysate (20ug), HEK293 whole cell lysate (20ug), NIH/3T3 whole cell lysate (20ug), All lanes: HSPA8 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 71 kDa, Observed band size: 71 kDa, Exposure time:10s
Western Blot, Positive WB detected in: Mouse brain tissue lysate (20ug), Mouse uterus tissue lysate (20ug), All lanes: HSPA8 antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 70.9 kDa, Observed band size: 71 kDa, Exposure time: 2min
IHC image of RAC08144 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF-7 cell with RAC08144 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L).
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HSPA8 Recombinant Monoclonal Antibody, Cat#RAC08144 Western Blot, Positive WB detected in: A549 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), U-251MG whole cell lysate (20ug), MCF-7 whole cell lysate (20ug), K562 whole cell lysate (20ug), HepG2 whole cell lysate (20ug), HeLa whole cell lysate (20ug), HEK293 whole cell lysate (20ug), NIH/3T3 whole cell lysate (20ug), All lanes: HSPA8 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 71 kDa, Observed band size: 71 kDa, Exposure time:10s Western Blot, Positive WB detected in: Mouse brain tissue lysate (20ug), Mouse uterus tissue lysate (20ug), All lanes: HSPA8 antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 70.9 kDa, Observed band size: 71 kDa, Exposure time: 2min IHC image of RAC08144 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with RAC08144 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L).

Ultra Sensitive IHC

HSPA8 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08144-100ul
Size: 100ul
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Western Blot, Positive WB detected in: A549 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), U-251MG whole cell lysate (20ug), MCF-7 whole cell lysate (20ug), K562 whole cell lysate (20ug), HepG2 whole cell lysate (20ug), HeLa whole cell lysate (20ug), HEK293 whole cell lysate (20ug), NIH/3T3 whole cell lysate (20ug), All lanes: HSPA8 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 71 kDa, Observed band size: 71 kDa, Exposure time:10s
Western Blot, Positive WB detected in: Mouse brain tissue lysate (20ug), Mouse uterus tissue lysate (20ug), All lanes: HSPA8 antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 70.9 kDa, Observed band size: 71 kDa, Exposure time: 2min
IHC image of RAC08144 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF-7 cell with RAC08144 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L).

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We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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Yue Home Liu

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The peptide product is of exceptional quality and is highly recommended for its efficacy.

Haoyang Xin

I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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