Hnrnpc Recombinant Antibody, Cat#RAC07411
Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate; All lanes: hnRNP C1 + C2 antibody at 0.66ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34, 33, 26, 28 KDa; Observed band size: 42 KDa
IHC image of RAC07411 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07411 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating hnRNP C1 + C2 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07411 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07411 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)
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Hnrnpc Recombinant Antibody, Cat#RAC07411 Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate; All lanes: hnRNP C1 + C2 antibody at 0.66ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34, 33, 26, 28 KDa; Observed band size: 42 KDa IHC image of RAC07411 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with RAC07411 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating hnRNP C1 + C2 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07411 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07411 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)

Ultra Sensitive IHC

Hnrnpc Recombinant Antibody

BiomatikSKU: RAC07411-100ul
Size: 100ul
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Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate; All lanes: hnRNP C1 + C2 antibody at 0.66ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34, 33, 26, 28 KDa; Observed band size: 42 KDa
IHC image of RAC07411 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07411 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating hnRNP C1 + C2 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07411 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07411 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)

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