Hdac6 Recombinant Antibody, Cat#RAC07393
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; All lanes: HDAC6 antibody at 1.1ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 132, 115 KDa; Observed band size: 160 KDa
IHC image of RAC07393 diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HDAC6 in HepG2 whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07393 in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07393 (3ug) + HepG2 whole cell lysate (500ug); Lane 3: HepG2 whole cell lysate (20ug)
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Hdac6 Recombinant Antibody, Cat#RAC07393 Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; All lanes: HDAC6 antibody at 1.1ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 132, 115 KDa; Observed band size: 160 KDa IHC image of RAC07393 diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating HDAC6 in HepG2 whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07393 in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07393 (3ug) + HepG2 whole cell lysate (500ug); Lane 3: HepG2 whole cell lysate (20ug)

Ultra Sensitive IHC

Hdac6 Recombinant Antibody

BiomatikSKU: RAC07393-100ul
Size: 100ul
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Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; All lanes: HDAC6 antibody at 1.1ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 132, 115 KDa; Observed band size: 160 KDa
IHC image of RAC07393 diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HDAC6 in HepG2 whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07393 in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07393 (3ug) + HepG2 whole cell lysate (500ug); Lane 3: HepG2 whole cell lysate (20ug)

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