GZMB Recombinant Monoclonal Antibody, Cat#RAC08069
Western Blot. Positive WB detected in:K562 whole cell lysate (20µg). All lanes: GZMB antibody at 1:1000. Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size:27.7 kDa. Observed band size:28 kDa. Exposure time:300s
IHC image of diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing THP-1 cells stained at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
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GZMB Recombinant Monoclonal Antibody, Cat#RAC08069 Western Blot. Positive WB detected in:K562 whole cell lysate (20µg). All lanes: GZMB antibody at 1:1000. Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size:27.7 kDa. Observed band size:28 kDa. Exposure time:300s IHC image of diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing THP-1 cells stained at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Ultra Sensitive IHC

GZMB Recombinant Monoclonal Antibody

BiomatikSKU: RAC08069-100ul
Size: 100ul
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Western Blot. Positive WB detected in:K562 whole cell lysate (20µg). All lanes: GZMB antibody at 1:1000. Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size:27.7 kDa. Observed band size:28 kDa. Exposure time:300s
IHC image of diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing THP-1 cells stained at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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The peptide product is of exceptional quality and is highly recommended for its efficacy.

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I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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