Western Blot; Positive WB detected in: U251 whole cell lysate, MCF7 whole cell lysate; All lanes: GSN antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 85 kDa; Observed band size: 85 kDa
Immunofluorescence staining of hela cell with RAC07882 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of NIH/3T3 cell with RAC07882 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).