GBP2 Recombinant Monoclonal Antibody, Cat#RAC08146
Western Blot, Positive WB detected in: U-251MG whole cell lysate (20ug), All lanes: GBP2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 67 kDa, Observed band size: 67 kDa, Exposure time:2min
Immunofluorescence staining of Hela cell with RAC08146 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L).
Overlay Peak curve showing A431 cells stained with RAC08146 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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GBP2 Recombinant Monoclonal Antibody, Cat#RAC08146 Western Blot, Positive WB detected in: U-251MG whole cell lysate (20ug), All lanes: GBP2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 67 kDa, Observed band size: 67 kDa, Exposure time:2min Immunofluorescence staining of Hela cell with RAC08146 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L). Overlay Peak curve showing A431 cells stained with RAC08146 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

GBP2 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08146-100ul
Size: 100ul
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Western Blot, Positive WB detected in: U-251MG whole cell lysate (20ug), All lanes: GBP2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 67 kDa, Observed band size: 67 kDa, Exposure time:2min
Immunofluorescence staining of Hela cell with RAC08146 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L).
Overlay Peak curve showing A431 cells stained with RAC08146 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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