Western Blot, Positive WB detected in: HEK293 whole cell lysate (20ug), U-251MG whole cell lysate (20ug), HeLa whole cell lysate (20ug), Raji whole cell lysate (20ug), A549 whole cell lysate (20ug), HepG2 whole cell lysate (20ug), All lanes: FLOT1 antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa, Exposure time:1min
Western Blot, Positive WB detected in: Rat brain tissue lysate (20ug), All lanes: FLOT1 antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 47.4 kDa, Observed band size: 48 kDa, Exposure time: 2min
Immunofluorescence staining of NIH/3T3 cell with RAC08151 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
Immunofluorescence staining of Hela cell with RAC08151 at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.