FGFR2 Recombinant Monoclonal Antibody, Cat#RAC08082
Western Blot. Positive WB detected in:hela whole cell lysate (20µg), HT29 whole cell lysate (20µg). All lanes: FGFR2 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:92.03, 86.13, 76.70, 28.29, 40.61, 79 kDa. Observed band size:41 kDa. Exposure time:120s
IHC image diluted at 1:100 and staining in paraffin-embedded performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
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FGFR2 Recombinant Monoclonal Antibody, Cat#RAC08082 Western Blot. Positive WB detected in:hela whole cell lysate (20µg), HT29 whole cell lysate (20µg). All lanes: FGFR2 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:92.03, 86.13, 76.70, 28.29, 40.61, 79 kDa. Observed band size:41 kDa. Exposure time:120s IHC image diluted at 1:100 and staining in paraffin-embedded performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

Ultra Sensitive IHC

FGFR2 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08082-100ul
Size: 100ul
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Images

Western Blot. Positive WB detected in:hela whole cell lysate (20µg), HT29 whole cell lysate (20µg). All lanes: FGFR2 antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:92.03, 86.13, 76.70, 28.29, 40.61, 79 kDa. Observed band size:41 kDa. Exposure time:120s
IHC image diluted at 1:100 and staining in paraffin-embedded performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

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