Western Blot; Positive WB detected in: SY5Y whole cell lysate,U251 whole cell lysate,MCF7 whole cell lysate,K562 whole cell lysate,Mouse Brain tissue lysate,Mouse Brain tissue lysate; All lanes: EGR2 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 50 kDa; Observed band size: 50 kDa
Overlay Peak curve showing SH-SY5Y cells stained with RAC07310 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.