Western Blot, Positive WB detected in: U-251MG whole cell lysate (30ug), U-87MG whole cell lysate (30ug), HEK293T whole cell lysate (30ug), A2780 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), Rat heart tissue lysate (30ug), Mouse skeletal muscle tissue lysate (30ug), Rat skeletal muscle tissue lysate (30ug), All lanes: CRYAB antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 20 kDa, Observed band size: 22 kDa, Exposure time:2min
IHC image of RAC08502 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.