Cops3 Recombinant Antibody, Cat#RAC07260
Western Blot; Positive WB detected in: U251 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; All lanes: COPS3 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 46 kDa; Observed band size: 40-55 kDa
IHC image of RAC07260 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07260 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with RAC07260 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 535-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Cops3 Recombinant Antibody, Cat#RAC07260 Western Blot; Positive WB detected in: U251 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; All lanes: COPS3 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 46 kDa; Observed band size: 40-55 kDa IHC image of RAC07260 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of RAC07260 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with RAC07260 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 535-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Ultra Sensitive IHC

Cops3 Recombinant Antibody

BiomatikSKU: RAC07260-100ul
Size: 100ul
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Western Blot; Positive WB detected in: U251 whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; All lanes: COPS3 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 46 kDa; Observed band size: 40-55 kDa
IHC image of RAC07260 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07260 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with RAC07260 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 535-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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