CHUK Recombinant Monoclonal Antibody, Cat#RAC08229
Western Blot, Positive WB detected in: HEK293 whole cell lysate (30ug), A549 whole cell lysate (30ug), A-431 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), HeLa whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), All lanes: IKK alpha antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 85 kDa, Observed band size: 85 kDa, Exposure time: 2min
Overlay Peak curve showing HepG2 cells stained with https://www.cusabio.com/uploadfile/antibody/Recombinant/RAC08229 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CHUK Recombinant Monoclonal Antibody, Cat#RAC08229 Western Blot, Positive WB detected in: HEK293 whole cell lysate (30ug), A549 whole cell lysate (30ug), A-431 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), HeLa whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), All lanes: IKK alpha antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 85 kDa, Observed band size: 85 kDa, Exposure time: 2min Overlay Peak curve showing HepG2 cells stained with https://www.cusabio.com/uploadfile/antibody/Recombinant/RAC08229 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

CHUK Recombinant Monoclonal Antibody

BiomatikSKU: RAC08229-100ul
Size: 100ul
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Western Blot, Positive WB detected in: HEK293 whole cell lysate (30ug), A549 whole cell lysate (30ug), A-431 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), HeLa whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), All lanes: IKK alpha antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 85 kDa, Observed band size: 85 kDa, Exposure time: 2min
Overlay Peak curve showing HepG2 cells stained with https://www.cusabio.com/uploadfile/antibody/Recombinant/RAC08229 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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