Western Blot, Positive WB detected in: A549 whole cell lysate (30ug), All lanes: CEACAM6 antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 37 kDa, Observed band size: 70 kDa, Exposure time: 60s
IHC image of RAC08842 diluted at 1:50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC08842 diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing BxPC-3 cells stained with RAC08842 (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
Safety Data Sheet