Western Blot, Positive WB detected in: K562 whole cell lysate (30ug), HL-60 whole cell lysate (30ug), Jurkat whole cell lysate (30ug), Hela whole cell lysate (30ug), HEK293T whole cell lysate (30ug), THP-1 whole cell lysate (30ug), SP2/0 whole cell lysate (30ug), NIH/3T3 whole cell lysate (30ug), All lanes: CDC40 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 66 kDa, Observed band size: 74 kDa, Exposure time:40s
IHC image of RAC08557 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.