IHC image of RAC08416 diluted at 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with RAC08416 at 1:30,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG (H+L).
Overlay Peak curve showing Raji cells surface stained with RAC08416 (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was mouse IgG2a (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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