Cd19 Recombinant Antibody, Cat#RAC07065
Western Blot; Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate; All lanes: CD19 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 62 kDa; Observed band size: 95 kDa;
IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD19 in Raji whole cell lysate; , Lane 1: Rabbit control IgG instead in Raji whole cell lysate., For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07065(2µg)+ Raji whole cell lysate(500µg); , Lane 3: Raji whole cell lysate (10µg);
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Cd19 Recombinant Antibody, Cat#RAC07065 Western Blot; Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate; All lanes: CD19 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 62 kDa; Observed band size: 95 kDa; IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunoprecipitating CD19 in Raji whole cell lysate; , Lane 1: Rabbit control IgG instead in Raji whole cell lysate., For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07065(2µg)+ Raji whole cell lysate(500µg); , Lane 3: Raji whole cell lysate (10µg);

Ultra Sensitive IHC

Cd19 Recombinant Antibody

BiomatikSKU: RAC07065-100ul
Size: 100ul
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Western Blot; Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate; All lanes: CD19 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 62 kDa; Observed band size: 95 kDa;
IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD19 in Raji whole cell lysate; , Lane 1: Rabbit control IgG instead in Raji whole cell lysate., For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07065(2µg)+ Raji whole cell lysate(500µg); , Lane 3: Raji whole cell lysate (10µg);

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