Cd147 Recombinant Antibody, Cat#RAC07835
The Binding Activity of CD147 with Anti-CD147 recombinant Antibody; Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD147 at 2 ug/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
Western Blot; Positive WB detected in: HepG2 whole cell lysate, ntera2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate ; All lanes: CD147 antibody at 1:1000; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 42, 29, 23, 19 KDa; Observed band size: 35, 50-60 KDa
IHC image of RAC07835 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07835 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Cd147 Recombinant Antibody, Cat#RAC07835 The Binding Activity of CD147 with Anti-CD147 recombinant Antibody; Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD147 at 2 ug/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml. Western Blot; Positive WB detected in: HepG2 whole cell lysate, ntera2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate ; All lanes: CD147 antibody at 1:1000; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 42, 29, 23, 19 KDa; Observed band size: 35, 50-60 KDa IHC image of RAC07835 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of RAC07835 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

Cd147 Recombinant Antibody

BiomatikSKU: RAC07835-100ul
Size: 100ul
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The Binding Activity of CD147 with Anti-CD147 recombinant Antibody; Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD147 at 2 ug/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
Western Blot; Positive WB detected in: HepG2 whole cell lysate, ntera2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate ; All lanes: CD147 antibody at 1:1000; Secondary; Goat polyclonal to mouse IgG at 1/50000 dilution; Predicted band size: 42, 29, 23, 19 KDa; Observed band size: 35, 50-60 KDa
IHC image of RAC07835 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07835 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

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