Western Blot, Positive WB detected in: Jurkat whole cell lysate (30ug), Raji whole cell lysate (30ug), A549 whole cell lysate (30ug), A431 whole cell lysate (30ug), K562 whole cell lysate (30ug), U-937 whole cell lysate (30ug), HL-60 whole cell lysate (30ug), Mouse lung tissue lysate (30ug), All lanes: CCR7 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 43 kDa, Observed band size: 43 kDa, Exposure time:20s
Immunofluorescence staining of A549 cell with RAC08675 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).