CCNH Recombinant Monoclonal Antibody, Cat#RAC08374
Western Blot, Positive WB detected in: SW620 whole cell lysate (30ug),HEK293 whole cell lysate (30ug), Raji whole cell lysate (30ug), Hela whole cell lysate (30ug),A549 whole cell lysate (30ug),K562 whole cell lysate (30ug),Mouse small intestine tissue lysate (30ug), All lanes: Cyclin H antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 38 kDa, Observed band size: 38 kDa, Exposure time:2min
IHC image of RAC08374 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of THP-1 cell with RAC08374 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing PC-3 cells stained with RAC08374 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CCNH Recombinant Monoclonal Antibody, Cat#RAC08374 Western Blot, Positive WB detected in: SW620 whole cell lysate (30ug),HEK293 whole cell lysate (30ug), Raji whole cell lysate (30ug), Hela whole cell lysate (30ug),A549 whole cell lysate (30ug),K562 whole cell lysate (30ug),Mouse small intestine tissue lysate (30ug), All lanes: Cyclin H antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 38 kDa, Observed band size: 38 kDa, Exposure time:2min IHC image of RAC08374 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of THP-1 cell with RAC08374 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay Peak curve showing PC-3 cells stained with RAC08374 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

Ultra Sensitive IHC

CCNH Recombinant Monoclonal Antibody

BiomatikSKU: RAC08374-100ul
Size: 100ul
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Western Blot, Positive WB detected in: SW620 whole cell lysate (30ug),HEK293 whole cell lysate (30ug), Raji whole cell lysate (30ug), Hela whole cell lysate (30ug),A549 whole cell lysate (30ug),K562 whole cell lysate (30ug),Mouse small intestine tissue lysate (30ug), All lanes: Cyclin H antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 38 kDa, Observed band size: 38 kDa, Exposure time:2min
IHC image of RAC08374 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of THP-1 cell with RAC08374 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing PC-3 cells stained with RAC08374 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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