The Binding Activity of Human CCL17 with Anti-CCL17 recombinant antibody, Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-CCL17 recombinant antibody at 2 ug/mL can bind Human CCL17 protein. The EC50 is 2.403-2.741 ng/mL.
The Binding Activity of Macaca mulatta CCL17 with Anti-CCL17 recombinant antibody, Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-CCL17 recombinant antibody at 2 ug/mL can bind Mouse Ccl17 protein. The EC50 is 0.9792-1.486 ng/mL.
The Binding Activity of Macaca mulatta CCL17 with Anti-CCL17 recombinant antibody, Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-CCL17 recombinant antibody at 2 ug/mL can bind Macaca mulatta CCL17 protein. The EC50 is 1.953-2.469 ng/mL.
Overlay Peak curve showing Raji cells stained with RAC08128 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.