Western Blot, Positive WB detected in: THP-1 whole cell lysate (30ug), A431 whole cell lysate (30ug), U-251MG whole cell lysate (30ug), HeLa whole cell lysate (30ug), HT-29 whole cell lysate (30ug), PC-3 whole cell lysate (30ug), MCF-7 whole cell lysate (30ug), K562 whole cell lysate (30ug), All lanes: CAP1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 52 kDa, Observed band size: 55 kDa, Exposure time:30s
IHC image of RAC08534 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.