Western Blot; Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate; All lanes: Bcl-XL antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 27, 19, 26 kDa; Observed band size: 30 kDa
IHC image of RAC07179 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.