Western Blot; Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate; All lanes: ATM antibody at 2.05µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 350 KDa; Observed band size: 350 KDa;
IHC image diluted at 1:205 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:205 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating ATM in PC3 whole cell lysate; , Lane 1: Rabbit control IgG instead in PC3 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07016 (3µg) + PC3 whole cell lysate (500µg); , Lane 3: PC3 whole cell lysate (20µg);