Atf4 Recombinant Antibody, Cat#RAC07020
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate; All lanes: ATF4 antibody at 1.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 39 KDa; Observed band size: 50 KDa;
IHC image diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with RAC07020 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ATF4 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07020 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg);
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Atf4 Recombinant Antibody, Cat#RAC07020 Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate; All lanes: ATF4 antibody at 1.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 39 KDa; Observed band size: 50 KDa; IHC image diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with RAC07020 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating ATF4 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07020 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg);

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Atf4 Recombinant Antibody

BiomatikSKU: RAC07020-100ul
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Size: 100ul
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Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate; All lanes: ATF4 antibody at 1.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 39 KDa; Observed band size: 50 KDa;
IHC image diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with RAC07020 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ATF4 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07020 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg);

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I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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