Ultra Sensitive IHC

ARHGDIA Recombinant Monoclonal Antibody

BiomatikSKU: RAC09104-100ul

Size: 100ul
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Description

Technical Documents

Images

Western Blot,, Positive WB detected in:A549 whole cell lysate (30ug), PC-3 whole cell lysate (30ug), MCF7 whole cell lysate (30ug), SH-SY5Y whole cell lysate (30ug), A431 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), Mouse lung tissue lysate (30ug), Rat lung lysate (30ug), All lanes: RhoGDI 1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 23 kDa, Observed band size: 25 kDa, Exposure time:60s
IHC image of RAC09104 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

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