Western Blot, All lanes: WNT4 antibody at 8ug/ml + MCF-7 whole cell lysate, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution, Predicted band size: 40, 33 kDa, Observed band size: 40 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CAC13999 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CAC13999 at dilution of 1:100
IHC image of CAC13999 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.