Ultra Sensitive IHC

Wfdc2 Polyclonal Antibody

BiomatikSKU: CAC09115-100ug

Size: 100ug
Request Information
!

This item is non-returnable unless there is a quality issue. See Returns Policy

Description

Technical Documents

Images

Western Blot; Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue; All lanes: WFDC2 antibody at 4µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 13, 9, 12 kDa; Observed band size: 13 kDa
IHC image diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of MCF-7 cells using CAC09115 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image diluted at 1:200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Recently viewed