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Western blot; All lanes: VPS25 antibody at 3µg/ml; Lane 1: Mouse kidney tissue; Lane 2: HL60 whole cell lysate; Lane 3: THP-1 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 21 kDa; Observed band size: 21 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CAC09896 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CAC09896 at dilution of 1:100
Immunofluorescence staining of Hela cells with CAC09896 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Safety Data Sheet