Western Blot; Positive WB detected in: Jurkat whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue, Mouse skeletal muscle tissue; All lanes: USE1 antibody at 2.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 30, 17, 16 kDa; Observed band size: 36 kDa;
Immunofluorescent analysis of U251 cells using CAC08154 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating USE1 in PC-3 whole cell lysate ; , Lane 1: Rabbit control IgG (1µg) instead in PC-3 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC08154 (6µg) + PC-3 whole cell lysate (500µg); , Lane 3: PC-3 whole cell lysate (10µg);