Western Blot, Positive WB detected in: Mouse heart tissue, All lanes: UBE2L3 antibody at 3ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 18, 15, 25 kDa, Observed band size: 18 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CAC15069 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC15069 at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CAC15069 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).