Western Blot, Positive WB detected in: A549 whole cell lysate, NIH/3T3 whole cell lysate, Mouse skeletal muscle tissue, All lanes: TRIM39 antibody at 3ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 60, 57 kDa, Observed band size: 45, 50 kDa
IHC image of CAC14797 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of Hela cells using CAC14797 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)