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Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-TP53 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.
Western Blot; ,Positive WB detected in: MCF-7 whole cell lysate, Rat brain tissue, Mouse brain tissue; ,All lanes: TP53 antibody at 2.5µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa; ,Observed band size: 44 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CAC07006 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CAC07006 at dilution of 1:100

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