Immunofluorescence staining of Hela cells with CAC14106 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human heart tissue using CAC14106 at dilution of 1:100
Immunoprecipitating TNNI3 in Rat heart tissue, Lane 1: Rabbit control IgG instead of CAC14106 in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000), Lane 2: CAC14106 (8ug) + Rat heart tissue (500ug), Lane 3: Rat heart tissue (20ug)