Tnf Polyclonal Antibody

BiomatikSKU: CAC07005-100ug

Size: 100ug
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Description

Technical Documents

Images

Western Blot; Positive WB detected in: Hela whole cell lysate; All lanes: TNF antibody at 3.2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 26 kDa; Observed band size: 26 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CAC07005 at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CAC07005 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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