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Western Blot, Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, All lanes: TMEM214 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 78, 73 kDa, Observed band size: 78 kDa
Immunoprecipitating TMEM214 in HepG2 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15766 in HepG2 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC15766 (5?g) + HepG2 whole cell lysate (0.5mg), Lane 3: HepG2 whole cell lysate (20?g)

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