IHC image of CAC11860 diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC11860 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; , All lanes: SULT1A3 antibody at 20µg/ml; , Lane 1: A549 whole cell lysate; , Lane 2: Colo320 whole cell lysate; , Lane 3: Mouse brain tissue; , Lane 4: Mouse kidney tissue; , Secondary; , Goat polyclonal to rabbit IgG at 1/10000 dilution; , Predicted band size: 35, 23 kDa; , Observed band size: 35 kDa