ST5 Polyclonal Antibody

BiomatikSKU: CAC15787-100ul

Size: 100ul
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Technical Documents

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IHC image of CAC15787 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot, Positive WB detected in: HepG2 whole cell lysate, U87 whole cell lysate, All lanes: ST5 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 127, 83, 71 kDa, Observed band size: 127 kDa

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