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IHC image of CAC15785 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot, Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, All lanes: SRPK2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 78, 80 kDa, Observed band size: 78 kDa

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