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Western blot; All lanes: SOD1 antibody at 6µg/ml; Lane 1: Mouse liver tissue; Lane 2: Mouse brain tissue; Lane 3: Hela whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: A549 whole cell lysate; Lane 6: HepG2 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 16 kDa; Observed band size: 16 kDa
IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of 293T cells using CAC07037 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Safety Data Sheet