Immunohistochemistry of paraffin-embedded human skin tissue using CAC14179 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CAC14179 at dilution of 1:100
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, All lanes: SMAD1 antibody at 3ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 53, 16 kDa, Observed band size: 53 kDa
IHC image of CAC14179 diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.