Ultra Sensitive IHC

RPL7A Polyclonal Antibody

BiomatikSKU: CAC15654-100ug

Size: 100ug
Request Information
!

This item is non-returnable unless there is a quality issue. See Returns Policy

Description

Technical Documents

Images

Western Blot, Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, All lanes: RPL7A antibody at 5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 30 kDa, Observed band size: 30 kDa
IHC image of CAC15654 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Recently viewed