Western Blot; Positive WB detected in: Rat brain tissue; All lanes: RPE65 antibody at 2.3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 61 kDa; Observed band size: 61 kDa;
Immunofluorescence staining of Hela cells with CAC11395 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; , Positive WB detected in: MCF-7 whole cell lysate; , All lanes: RPE65 antibody at 1:2000; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 61 kDa; , Observed band size: 61 kDa;