Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: RPA2 antibody at 2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 30, 31, 39 kDa, Observed band size: 30 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CAC13840 at dilution of 1:100
Immunofluorescent analysis of Hela cells using CAC13840 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating RPA2 in Hela whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC13840 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC13840 (6ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (10ug)