Ripk1 Polyclonal Antibody

BiomatikSKU: CAC09275-100ug

Size: 100ug
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Technical Documents

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Western Blot; Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat liver tissue, Mouse heart tissue, Mouse kidney tissue; All lanes: RIPK1 antibody at 3.2µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 76, 71 kDa; Observed band size: 76 kDa
Immunofluorescence staining of HepG2 cells with CAC09275 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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