Western Blot, Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, Rat brain tissue, All lanes: RFNG antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 37 kDa, Observed band size: 37 kDa
IHC image of CAC13553 diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.